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Ohio Plant Biotechnology Consortium

Member Institutions:

Randy Scholl

Associate Professor
Department of Plant Biology
The Ohio State University
Room 363 Botany and Zoology Building
1735 Neil Avenue
Columbus, Ohio 43210
Phone: Office (614) 292-1982, Lab (614) 292-9371
email scholl.1@osu.edu

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Randy Scholl

Research Interests

Ribosomal protein (RP) genes have been studied extensively in E. Coli, yeast and animals. Many genetic traits are associated with RPs: Resistance to trichothecene mycotoxins is conferred by ribosomal protein L3 (RPL3) of yeast, and the minute phenotype of Drosophila is the result of mutant RPs. Since trichothecenes are produced by some plant pathogenic fungi, we are studying Arabidopsis ribosomal protein L3 (ARP). Our previous studies have shown that there are three members of the ARP family, one of which (ARP1) is very abundant at the RNA level in all studied tissues, one of which (ARP2) is rarer and one (ARP3) which is a processed pseudogene. The translated sequences of ARP1 and ARP2 are more divergent than expected for members of the same RP gene family. In addition, the mRNA of ARP2 occurs largely in the poly(A)- fraction of all tissues examined, which is often associated with regulatory effects. Poly(A)- localization of ARP2 mRNA was shown both by RNA hybridization and comparative RT-PCR.

ARP2 is shown to be translated by polysome analysis. Assessment of whether the translated ARP2 mRNA is polyadenylated is being conducted. While the major portion of the ARP2 message pool is not polyadenylated, the ARP2 RNA found on polysomes is polyadenylated. The extent and circumstances of expression of gene family members are currently being further evaluated.

Phenotypic effects of ARPs are being studied via reverse genetics using T-DNA insertions, Ds transposition and site-directed mutagenesis. The former two approaches are being utilized to acquire knockout mutations of the two functional family members.

Selected References

  • Wang, X., K. A. Feldmann, and R. L. Scholl. 1988. A chlorate-hypersensitive, high nitrate; chlorate uptake mutant of Arabidopsis thaliana. Physiol. Plantar. 73: 305-310.
  • Kim, Y., H. Zhang and R.L. Scholl. 1990. Two evolutionarily divergent genes encode a cytoplasmic ribosomal protein of Arabidopsis thaliana. Gene 92:177-182.
  • Scholl, R.L. and K.A. Feldmann. 1990. Arabidopsis thaliana (L.): In vitro production of haploids. in: Biotechnology in Agriculture and Forestry vol. 12, Haploids in Crop Improvement I (ed. Y.P.S. Bajaj). Springer-Verlag, Berlin (pp. 309-320).
  • Griffing, B. and R.L. Scholl. l991. Qualitative and quantitative studies of Arabidopsis thaliana. Genetics 129:605-609.
  • Scholl, R., K. A. Feldmann and A. Paterson. 1994. Quantitative Genetics. in: E. M. Meyerowitz and C. R. Somverville (eds.). Arabidopsis. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. pp. 121-136; invited.
  • Scholl, R. , L. Rivero-Lepinckas and D. Crist. 1997. Growth of Plant and Preservation of Seeds. In: J. M. Martinez-Zapater and J. Salinas, eds. Methods in Molecular Biology; Arabidopsis Protocols 82:1-12. (Humana Press, Totowa, NJ.).
  • You T. H., and Scholl, R. L. 1998. PCR amplification of cDNA Libraries for Cloning and Screening. BioTechniques 24:575-575.

Recent Abstracts

  • Mary Anderson, R. Scholl, K. Davis. Arabidopsis Biological Resource Centers. Booth at Fifth International Conference on Araidopsis Reseach, OSU. August, 1993.
  • R. Scholl*, K. Davis. June 1994.Arabidopsis Biological Resource Centers. Mary Anderson, International Society for Plant Molecular Boilogy Conference, Amsterdam, The Netherlands,
  • Mary Anderson*, R. Scholl*, Bernard Mulligan, Keith Davis. 1994.Arabidopsis Biological Resource Centers. Plant Genome II Conference. San Diego, January, 1994.
  • R. Scholl*, K, and Sakti Pramanik. June 1994. The Arabidpsis Biological Resource Center and AIMS. Computer Science Dept., Michigan State U.), Invited presentation at the International Society for Plant Molecular Boilogy Conference, Amsterdam, The Netherlands.
  • You, T. H.*, R. L. Scholl. 1995.Molecular Biology of Ribosomal Proteins in Arabidopsis. 6th International Conference on Arabidopsis Research. Madison, WI. June.
  • Scholl, R. L. K. R. Davis. and S. Pramanik. 1996. The Arabidopsis Biological Resource Center and the AIMS Database. 7th International Conference on Arabidopsis Research, Norwich, U.K. June 1996. Tilley, M.* and R. Scholl. 1997. Regulation of Ribosomal Protein Gene Expression. 8th Annual Conference on Arabidopsis Research, Madison, WI.
  • Scholl, R.*, K. Davis. D. Ware and D. K. Crist. 1997. Update of ABRC Activities. 8th Annual Conference on Arabidopsis Research, Madison, WI.
  • Scholl, R.*, D. Ware, D. Crist and K. Davis. 1997. Assessment of the PCR-based screening of DNAs from pools of T-DNA lines shared through the ABRC. Arabidopsis Genome Conference. Cold Spring Harbor Laboratory, NY, Dec. 11-14.

Recent Invited Presentations

  • Scholl, R. L. Genetic Resources Available for Arabidopsis Research. Midwestern Plant Developmental Biology Meetings. Dayton, OH. May, 1992.
  • Scholl, R., K. Davis and S. Pramanik. 1994. The Arabidopsis Information Management System. Plant Genome II Conference. San Diego, CA.
  • Scholl, R. L. Arabidopsis germplasm. Lecture at Arabidopsis Course, Cold Spring Harbor Laboratory; July, 1995.
  • Scholl, R. L. 1995. Computer Resources for Arabidopsis Researchers. Laboratory demonstration, Course, Cold Spring Harbor Laboratory; July, 1995.
  • Scholl., R. L. Regulation of Ribosomal Protein Gene Expression in Arabidopsis. University of Toledo. April, 1995.
  • Scholl. R. L. 1996. The Arabidopsis Biological Resource Center and the AIMS Database. 7th International Conference on Arabidopsis Research. Norwich, U.K., June, 1996.

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