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Ohio Plant Biotechnology Consortium

Member Institutions:

Steven H. Rogstad

Associate Professor
University of Cincinnati
Department of Biological Sciences
ML006
Cincinnati, Ohio 45221-0006
Phone (513) 556-9744
FAX (513) 556-5299
email Steven.Rogstad@UC.Edu

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Research Interests

I am: 1) continuing to develop probes that reveal variable-hypervariable, minisatellite DNA lociacross a broad range of eukaryotes; and 2) investigating the utilization of these probes to examine a range of population biology phenomena in several plant species, including investigating how anthropogenic influences, especially environmental pollutants, affect population genetics and mutation rates in Taraxacum officinale (dandelions; Asteraceae), Typha latifolia (cattails; Typhaceae) and Rubus ideaus (wild raspberry; Rosaceae). In addition, I am investigating levels of outcrossing in a tropical tree (Polyalthia lateriflora; Annonaceae), as well as conducting research into clonal and population genetic variation in Quercus gambelii (Fagaceae), Aesculus glabra (Hippocastanaceae), Lindera benzoin (Lauraceae), and Rubus moluccanus (Rosaceae).

CURRENT FUNDING

P.I. on: Ecosystem Monitoring Via Genetic Diversity Surveys of Dandelions Using VNTR Multilocus DNA Probes; U.S. E.P.A. Assistance ID. No. R826602-01-0; $291,000 through Sept. 2001. We have demonstrated a method that can readily detect high mutation rates in dandelions at variable-number-tandem-repeat (VNTR) loci. We will explore whether mutation rates at dandelion VNTR loci differ in populations at severely polluted sites versus at relatively pristine sites.

Selected Publications

  • Rogstad, S. H., J. C. Patton, and B. A. Schaal. 1988a. M13 repeat probe detects DNA minisatellite-like sequences in gymnosperms and angiosperms. Proceedings of the National Academy of Science USA 85: 9176-9178.
  • Rogstad, S. H., J. C. Patton, and B. A. Schaal. 1988b. A human minisatellite probe reveals RFLPs among individuals of two angiosperms. Nucleic Acids Research 16: 11378.
  • Nybom, H., B. A. Schaal, and S. H. Rogstad. 1989. DNA "fingerprints" can distinguish cultivars of blackberries and raspberries. Acta Horticulturae 262: 305-310.
  • Rogstad, S. H., B. L. Herwaldt, P. H. Schlesinger, and D. J. Krogstad. 1989. The M13 repeat probe detects RFLPs between two strains of the protozoan malaria parasite Plasmodium falciparum. Nucleic Acids Research 17: 3610.
  • Nybom, H., S. H. Rogstad, and B. A. Schaal. 1990. Genetic variation detected by use of the M13 "DNA fingerprinting" probe in Malus, Prunus, and Rubus (Rosaceae). Theoretical and Applied Genetics 79: 153-156.
  • Nybom, H., and S. H. Rogstad. 1990. DNA "fingerprints" detect genetic variation in Acer Negundo (Aceraceae). Plant Systematics and Evolution 173: 49-56.
  • Rogstad, S. H. 1990. The biosystematics and evolution of the Polyalthia hypoleuca species complex (Annonaceae) of Malesia. II. Comparative distributional ecology. Journal of Tropical Ecology 6: 387-408.
  • Rogstad, S. H., H. Nybom, and B. A. Schaal. 1991. The tetrapod "DNA fingerprinting" M13 repeat probe reveals genetic diversity and clonal growth in quaking aspen (Populus tremuloides). Plant Systematics and Evolution 175: 115-123.
  • Rogstad, S. H., K. Wolff, and B. A. Schaal. 1991. Geographical variation in Asimina triloba Dunal (Annonaceae) revealed by the M13 "DNA fingerprinting" probe. American Journal of Botany. 78: 1391-1396.
  • Rogstad, S. H. 1993. Surveying plant genomes for variable number of tandem repeats loci. Methods in Enzymology 224: 278-294.
  • Rogstad, S. H. 1994. The biosystematics and evolution of the Polyalthia hypoleuca species complex (Annonaceae) of Malesia. III. Floral ontogeny and breeding systems. American Journal of Botany 81: 145-154.
  • Wolff, K., S. H. Rogstad, and B. A. Schaal. 1994. Population and species variation of minisatellite DNA in Plantago. Theoretical and Applied Genetics 87: 733-740.
  • Rogstad, S. H. 1994. Inheritance in turnip of variable number tandem repeat genetic markers revealed with synthetic repetitive DNA probes. Theoretical and Applied Genetics 89: 824-830.
  • Rogstad, S. H. 1996. Assessing genetic diversity in plants with synthetic tandem repetitive DNA probes. In, Genomes of Plants and Animals, J. P. Gustafson and R. B. Flavell, editors, Plenum Press, New York, New York. Pp. 1-14.
  • Rogstad, S. H., and S. Pelikan. 1996. GELSTATS: a computer program for population genetics analyses using VNTR multilocus probe data. BioTechniques 21: 1128-1131.
  • Busemeyer, D. T., S. Pelikan, R. S. Kennedy, and S. H. Rogstad. 1997. Genetic diversity of Philippine Rubus moluccanus L. (Rosaceae) populations compared with synthetic DNA VNTR loci probes. Journal of Tropical Ecology 14: 867-884.
  • Keane, B., M. K. Smith, and S. H. Rogstad. In press 1998. Genetic variation in red raspberries (Rubus idaeus L.; Rosaceae) from sites differing in organic pollutants compared with synthetic tandem repeat DNA probes. Environmental Toxicology and Chemistry Vol. 17.
  • Kumar, A., and S. H. Rogstad. In press 1998. A hierarchical analysis of minisatellite DNA genetic diversity in Gambel oak (Quercus gambelii Nutt.; Fagaceae). Molecular Ecology Vol. 7.
  • Pelikan, S. and S. H. Rogstad. Provisionally in press, pending minor modifications. Improved estimation of heterozygosity from VNTR multilocus probe data. Molecular Biology and Evolution.
  • Lim, H. W., S. Pelikan, and S. H. Rogstad. In press. Genetic diversity among populations and size classes of buckeyes (Aesculus: Hippocastanaceae) examined with multilocus VNTR DNA probes. Plant Systematics and Evolution.

Dr. Rogstad's website
University of Cincinnati Department of Biological Sciences
University of Cincinnati homepage