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David K. Asch Assistant Professor |
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Research Interests
I am primarily interested in the way that genes are turned on and off in response to different nutrient sources. In my laboratory we work with two different gene systems. One is the quinic acid gene cluster in the fungus Neurospora crassa. The other is the thymidine salvage pathway which is present in several different fungi including N. crassa. Our thymidine salvage projects are done in collaboration with Dr. Jeff Smiley in the department of Chemistry. In my laboratory students do basic molecular techniques such as gene cloning, gene sequencing and the polymerase chain reaction (PCR).
Selected References
- Smiley, J. A., J. M. Angelot, R. C. Cannon, E. M. Marshall, and D. K. Asch. 1998. Radioactivity-Based and Spectrophotometric Assays for Isoorotate Decarboxylase: Identification of the Thymidine Salvage Pathway in Lower Eukaryotes. Analytical Biochemistry 266:85-92.
- Asch, D.K., M. Orejas, R. F. Geever, and M. E. Case. 1991. Comparative studies of the quinic acid (qa) cluster in several Neurospora species with special emphasis on the qa-x-qa-2 intergenic region. Mol. Gen. Genet. 230: 337-344.
- Asch, D.K., G. Frederick, J. A.Kinsey, and D. D. Perkins. 1992. Analysis of junction sequences resulting from non-homologous recombination in Neurospora crassa. Genetics 130: 737-748.
- Case, M. E., R. F. Geever, and D. K. Asch. 1992. Use of gene replacement transformation to elucidate gene function in the qa cluster of Neurospora crassa. Genetics 130: 729-736.
- Perkins, D. D., J. A. Kinsey, D. K. Asch, and G. Frederick. 1993 Chromosome rearrangements recovered following transformation in Neurospora crassa. Genetics 134: 729-736.
Youngstown State Department of Biological
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